Human Angiogenesis Array G1000

Update：2014/7/20 20:30:16 View：2501

Type AAH-ANG-G1000
Description
Raybiotech Antibody Arrays

Introduction

Product Features

High sensitivity and specificity
Low sample volume (10-100 µl per array)
Large dynamic range of detection
Compatible with most sample types
Test 4 or 8 samples on each slide
Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Angiogenin	Angiopoietin-1	Angiopoietin-2	Angiosestatin	bFGF
EGF	ENA-78 (CXCL5)	Endostatin	GCSF	GM-CSF
GRO alpha/beta/gamma	I-309 (TCA-3/CCL1)	IFN-gamma	IGF-1	IL-10
IL-1 alpha (IL-1 F1)	IL-1 beta (IL-1 F2)	IL-2	IL-4	IL-6
IL-8 (CXCL8)	I-TAC (CXCL11)	Leptin	MCP-1 (CCL2)	MCP-3 (MARC/CCL7)
MCP-4 (CCL13)	MMP-1	MMP-9	PDGF-BB	PECAM-1 (CD31)
PLGF	RANTES (CCL5)	TGF beta 1	Thrombopoietin (TPO)	Tie-2
TIMP-1	TIMP-2	TNF alpha	uPAR	VEGF-A
VEGFR2	VEGFR3	VEGF-D

Species Detected

Human

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Angiogenesis Antibody Array G1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Lysis Buffer
Adhesive Plastic Strips
30 ml Centrifuge Tube
Manual

Other Materials Required

Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

Protocol Outline

Dry the glass slide
Block array surface
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.