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      白细胞分化抗原244ELISA试剂盒

      更新 ：2014/7/23 14:37:46      点击：2247
      • 产品型号   ELH-2B4
      • 产品描述

        描述

      产品介绍

      Product Features

      • Strip plates and additional reagents allow for use in multiple experiments
      • Quantitative protein detection
      • Establishes normal range
      • The best products for confirmation of antibody array data

      Target Name/s

      2B4

      Species Detected

      Human

      Application Notes

      Kit Components
      • Pre-Coated 96-well Strip Microplate
      • Wash Buffer
      • Stop Solution
      • Assay Diluent(s)
      • Lyophilized Standard
      • Biotinylated Detection Antibody
      • Streptavidin-Conjugated HRP
      • TMB One-Step Substrate
      Other Materials Required
      • Distilled or deionized water
      • Precision pipettes to deliver 2 µl to 1 µl volumes
      • Adjustable 1-25 µl pipettes for reagent preparation
      • 100 µl and 1 liter graduated cylinders
      • Tubes to prepare standard and sample dilutions
      • Absorbent paper
      • Microplate reader capable of measuring absorbance at 450nm
      • Log-log graph paper or computer and software for ELISA data analysis
      Protocol Outline
      1. Prepare all reagents, samples and standards as instructed in the manual.
      2. Add 100 µl of standard or sample to each well.
      3. Incubate 2.5 h at RT or O/N at 4°C.
      4. Add 100 µl of prepared biotin antibody to each well.
      5. Incubate 1 h at RT.
      6. Add 100 µl of prepared Streptavidin solution to each well.
      7. Incubate 45 min at RT.
      8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
      9. Incubate 30 min at RT.
      10. Add 50 µl of Stop Solution to each well.
      11. Read at 450 nm immediately.

      Specificity

      The antibody pair provided in this kit recognizes human 2B4/CD244/SLAMF4.

      Storage/Stability

      May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.

       

      Usage

      This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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