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      小鼠细胞因子抗体芯片2000

      更新 :2014/7/23 10:23:27      点击:2901
      • 产品型号   AAM-CYT-2000
      • 产品描述

        Raybiotech Products

      产品介绍

      Product Features

      • Easy to use
      • No specialized equipment needed
      • Compatible with nearly any liquid sample
      • Proven technology (many publications)
      • Highly sensitive (pg/ml)
      • Sandwich ELISA specificity
      • Higher density than ELISA, Western blot or bead-based multiplex

      Target Names

      Scroll over each target protein for more information

      4-1BB
      (TNFRSF9/CD137)
      6Ckine
      (CCL21)
      ACE
      ALK-1
      Amphiregulin
      Axl
      bFGF
      BLC
      (CXCL13)
      Cardiotrophin-1
      (CT-1)
      CD27
      (TNFRSF7)
      CD27 Ligand
      (TNFSF7)
      CD30 Ligand
      (TNFSF8)
      CD30
      (TNFRSF8)
      CD36
      (SR-B3)
      CD40
      (TNFRSF5)
      CD40 Ligand
      (TNFSF5)
      Chordin
      CRG-2
      CTACK
      (CCL27)
      CTLA-4
      (CD152)
      CXCL16
      Decorin
      DKK-1
      CD26
      (DPPIV)
      Dtk
      E-Cadherin
      EGF
      Endoglin
      (CD105)
      Eotaxin-1
      (CCL11)
      Eotaxin-2
      (MPIF-2/CCL24)
      Epigen
      Epiregulin
      E-Selectin
      Fas Ligand
      (TNFSF6)
      Fc gamma RIIB
      (CD32b)
      Flt-3 Ligand
      Fractalkine
      (CX3CL1)
      Galectin-1
      GCSF
      GITR
      (TNFRSF18)
      GITR Ligand
      (TNFSF18)
      GM-CSF
      Granzyme B
      Gas 1
      Gas 6
      HAI-1
      HGF
      HGFR
      ICAM-1
      (CD54)
      IFN-gamma
      IGFBP-2
      IGFBP-3
      IGFBP-5
      IGFBP-6
      IGF-1
      IGF-2
      IL-1 beta
      (IL-1 F2)
      IL-1 R4
      (ST2)
      IL-10
      IL-11
      IL-12 p40/p70
      IL-12 p70
      IL-13
      IL-15
      IL-17A
      IL-17B
      IL-17 RB
      IL-17E
      (IL-25)
      IL-17F
      IL-1 alpha
      (IL-1 F1)
      IL-1 RA
      (IL-1 F3)
      IL-2
      IL-2 R alpha
      IL-20
      IL-21
      IL-28A
      (IFN-lambda 2)
      IL-3
      IL-3 R beta
      IL-4
      IL-5
      IL-6
      IL-6 R
      IL-7
      IL-9
      I-TAC
      (CXCL11)
      JAM-A
      (CD321/F11R)
      KC
      (CXCL1)
      Leptin
      Leptin R
      LIX
      L-Selectin
      (CD62L)
      Lungkine
      (CXCL15)
      Lymphotactin
      (XCL1)
      MAdCAM-1
      MCP-1
      (CCL2)
      MCP-5
      M-CSF
      MDC
      (CCL22)
      MFG-E8
      MIG
      (CXCL9)
      MIP-1 alpha
      (CCL3)
      MIP-1 gamma
      MIP-2
      MIP-3 beta
      (CCL19)
      MIP-3 alpha
      (CCL20)
      MMP-2
      MMP-3
      Neprilysin
      Osteopontin
      (SPP1)
      Osteoprotegerin
      (TNFRSF11B)
      Pentraxin-3
      (TSG-14)
      Platelet Factor 4
      (CXCL4)
      Prolactin
      Pro-MMP-9
      P-Selectin
      RAGE
      RANTES
      (CCL5)
      Resistin
      SCF
      SDF-1 alpha
      (CXCL12 alpha)
      Sonic Hedgehog N-Terminal
      (Shh-N)
      TNF RI
      (TNFRSF1A)
      TNF RII
      (TNFRSF1B)
      TACI
      (TNFRSF13B)
      TARC
      (CCL17)
      I-309
      (TCA-3/CCL1)
      TECK
      (CCL25)
      TCK-1
      (CXCL7)
      TIMP-1
      TIMP-2
      TNF alpha
      Thrombopoietin
      (TPO)
      TRANCE
      (TNFSF11)
      TREM-1
      TROY
      (TNFRSF19)
      TSLP
      TWEAK
      (TNFSF12)
      TWEAK R
      (TNFRSF12)
      VCAM-1
      (CD106)
      VEGF-A
      VEGFR1
      VEGFR2
      VEGFR3
      VEGF-D

      Species Detected

      Mouse

      Application Notes

      Suggested Application
      Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
      Kit Components
      • Mouse Cytokine Antibody Array C2000 Membranes
      • Blocking Buffer
      • Wash Buffer 1
      • Wash Buffer 2
      • Biotinylated Detection Antibody Cocktail
      • Streptavidin-Conjugated HRP
      • Detection Buffer C
      • Detection Buffer D
      • Lysis Buffer
      • 8-Well Incubation Tray
      • Plastic Sheets
      • Array Templates
      • Manual
      Other Materials Required
      • Pipettors, pipet tips and other common lab consumables
      • Orbital shaker or oscillating rocker
      • Tissue Paper, blotting paper or chromatography paper
      • Adhesive tape or Saran Wrap
      • Distilled or de-ionized water
      • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
      Protocol Outline
      1. Block membranes
      2. Incubate with Sample
      3. Incubate with Biotinylated Detection Antibody Cocktail
      4. Incubate with HRP-Conjugated Streptavidin
      5. Incubate with Detection Buffers
      6. Image with chemiluminescent imaging system
      7. Perform densitometry and analysis

      Storage/Stability

      For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

      Number of Targets Detected

      144

       

      Usage

      This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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