小鼠细胞因子抗体芯片5
更新 ：2014/7/21 17:26:37 点击：2777
- 产品型号 AAM-CYT-5
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
4-1BB
(TNFRSF9/CD137) |
6Ckine
(CCL21) |
ACE
|
ALK-1
|
Amphiregulin
|
|
Cardiotrophin-1
(CT-1) |
CD27
(TNFRSF7) |
CD27 Ligand
(TNFSF7) |
CD36
(SR-B3) |
CD40 Ligand
(TNFSF5) |
|
Chordin
|
CTLA-4
(CD152) |
Decorin
|
DKK-1
|
E-Cadherin
|
|
EGF
|
Endoglin
(CD105) |
Epigen
|
Epiregulin
|
Galectin-1
|
|
Gas 1
|
Gas 6
|
GITR Ligand
(TNFSF18) |
Granzyme B
|
HAI-1
|
|
HGF
|
IL-1 R4
(ST2) |
IL-11
|
IL-17B
|
IL-17E
(IL-25) |
|
IL-17F
|
IL-1 RA
(IL-1 F3) |
IL-2 R alpha
|
IL-20
|
IL-21
|
|
IL-28A
(IFN-lambda 2) |
IL-6 R
|
JAM-A
(CD321/F11R) |
MAdCAM-1
|
MFG-E8
|
|
Neprilysin
|
Pentraxin-3
(TSG-14) |
Prolactin
|
RAGE
|
TACI
(TNFRSF13B) |
|
TREM-1
|
TWEAK
(TNFSF12) |
TWEAK R
(TNFRSF12) |
||
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
48
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
更多产品
