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      小鼠细胞因子抗体芯片2

      更新:2014/7/21 17:03:31      点击:1927
      • 产品型号   AAM-CYT-2
      • 产品描述

        Raybiotech Products


      产品介绍

      Product Features

      • Easy to use
      • No specialized equipment needed
      • Compatible with nearly any liquid sample
      • Proven technology (many publications)
      • Highly sensitive (pg/ml)
      • Sandwich ELISA specificity
      • Higher density than ELISA, Western blot or bead-based multiplex

      Target Names

      Scroll over each target protein for more information

      6Ckine
      (CCL21)
      CTACK
      (CCL27)
      Eotaxin-1
      (CCL11)
      GCSF
      GM-CSF
      IL-2
      IL-3
      IL-4
      IL-5
      IL-6
      IL-9
      IL-10
      IL-12 p40/p70
      IL-12 p70
      IL-13
      IL-17A
      IFN-gamma
      KC
      (CXCL1)
      Leptin
      MCP-1
      (CCL2)
      MCP-5
      MIP-1 alpha
      (CCL3)
      MIP-2
      MIP-3 beta
      (CCL19)
      RANTES
      (CCL5)
      SCF
      TNF RI
      (TNFRSF1A)
      TARC
      (CCL17)
      TIMP-1
      TNF alpha
      Thrombopoietin
      (TPO)
      VEGF-A

      Species Detected

      Mouse

      Application Notes

      Suggested Application
      Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
      Kit Components
      • Mouse Cytokine Antibody Array C2 Membranes
      • Blocking Buffer
      • Wash Buffer 1
      • Wash Buffer 2
      • Biotinylated Detection Antibody Cocktail
      • Streptavidin-Conjugated HRP
      • Detection Buffer C
      • Detection Buffer D
      • Lysis Buffer
      • 8-Well Incubation Tray
      • Plastic Sheets
      • Array Templates
      • Manual
      Other Materials Required
      • Pipettors, pipet tips and other common lab consumables
      • Orbital shaker or oscillating rocker
      • Tissue Paper, blotting paper or chromatography paper
      • Adhesive tape or Saran Wrap
      • Distilled or de-ionized water
      • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
      Protocol Outline
      1. Block membranes
      2. Incubate with Sample
      3. Incubate with Biotinylated Detection Antibody Cocktail
      4. Incubate with HRP-Conjugated Streptavidin
      5. Incubate with Detection Buffers
      6. Image with chemiluminescent imaging system
      7. Perform densitometry and analysis

      Storage/Stability

      For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

      Number of Targets Detected

      32

       

      Usage

      This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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