人细胞因子抗体芯片10
更新：2014/7/21 12:47:24 点击 ：1810
- 产品型号 AAH-CYT-10
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
4-1BB
(TNFRSF9/CD137) |
ACE-2
|
Alpha-fetoprotein
|
Angiopoietin-1
|
Angiosestatin
|
|
ANGPTL4
|
Beta-2 Microglobulin
|
BCMA
(TNFRSF17) |
beta IG-H3
|
CA125
|
|
CA15-3
|
CA19-9
|
CA9
|
Cathepsin S
|
HCC-1
(CCL14) |
|
6Ckine
(CCL21) |
CD23
|
CD40 Ligand
(TNFSF5) |
CEA
|
CEACAM-1
|
|
Cripto-1
|
CRP
(C-Reactive Protein) |
DAN
|
Decorin
|
DKK-1
|
|
Dkk-3
|
Dkk-4
|
CD26
(DPPIV) |
E-Cadherin
|
EDA-A2
|
|
EG-VEGF
(PK1) |
TROP1
(EpCAM) |
ErbB2
|
Erythropoietin R
|
FSH
|
|
HB-EGF
|
hCG intact
|
HVEM
(TNFRSF14) |
IL-13 R1
|
IL-17B
|
|
IL-17C
|
IL-17F
|
IL-17 RA
|
Procalcitonin
|
PSA-free
|
|
S100 B
|
Sonic Hedgehog N-Terminal
(Shh-N) |
Thyroglobulin
|
Ubiquitin+1
|
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C10 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
49
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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