人细胞因子抗体芯片5
更新 ：2014/7/21 12:31:37 点击：1886
- 产品型号 AAH-CYT-5
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
ENA-78
(CXCL5) |
GCSF
|
GM-CSF
|
GRO alpha/beta/gamma
|
GRO alpha
(CXCL1) |
|
I-309
(TCA-3/CCL1) |
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
IL-2
|
IL-3
|
|
IL-4
|
IL-5
|
IL-6
|
IL-7
|
IL-8
(CXCL8) |
|
IL-10
|
IL-12 p40/p70
|
IL-13
|
IL-15
|
IFN-gamma
|
|
MCP-1
(CCL2) |
MCP-2
(CCL8) |
MCP-3
(MARC/CCL7) |
M-CSF
|
MDC
(CCL22) |
|
MIG
(CXCL9) |
MIP-1 beta
(CCL4) |
MIP-1 delta
(CCL15) |
RANTES
(CCL5) |
SCF
|
|
SDF-1 alpha
(CXCL12 alpha) |
TARC
(CCL17) |
TGF beta 1
|
TNF alpha
|
TNF beta
(TNFSF1B) |
|
EGF
|
IGF-1
|
Angiogenin
|
Oncostatin M
|
Thrombopoietin
(TPO) |
|
VEGF-A
|
PDGF-BB
|
Leptin
|
BDNF
|
BLC
(CXCL13) |
|
Ck beta 8-1
(CCL23) |
Eotaxin-1
(CCL11) |
Eotaxin-2
(MPIF-2/CCL24) |
Eotaxin-3
(CCL26) |
FGF-4
|
|
FGF-6
|
FGF-7
(KGF) |
FGF-9
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1) |
|
GCP-2
(CXCL6) |
GDNF
|
HGF
|
IGFBP-1
|
IGFBP-2
|
|
IGFBP-3
|
IGFBP-4
|
IL-16
|
IP-10
(CXCL10) |
LIF
|
|
Light
(TNFSF14) |
MCP-4
(CCL13) |
MIF
|
MIP-3 alpha
(CCL20) |
NAP-2
(PPBP/CXCL7) |
|
NT-3
|
NT-4
|
Osteopontin
(SPP1) |
Osteoprotegerin
(TNFRSF11B) |
PARC
(CCL18) |
|
PLGF
|
TGF beta 2
|
TGF beta 3
|
TIMP-1
|
TIMP-2
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C5 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
80
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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