人细胞因子抗体芯片4
更新 ：2014/7/21 12:29:22 点击 ：1927
- 产品型号 AAH-CYT-4
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
BDNF
|
BLC
(CXCL13) |
CK beta 8-1
(CCL23) |
Eotaxin-1
(CCL11) |
Eotaxin-2
(MPIF-2/CCL24) |
|
Eotaxin-3
(CCL26) |
FGF-4
|
FGF-6
|
FGF-7
(KGF) |
FGF-9
|
|
Flt-3 Ligand
|
Fractalkine
(CX3CL1) |
GCP-2
(CXCL6) |
GDNF
|
HGF
|
|
IGFBP-1
|
IGFBP-2
|
IGFBP-3
|
IGFBP-4
|
IL-16
|
|
LIF
|
LIGHT
(TNFSF14) |
MCP-4
(CCL13) |
MIF
|
NAP-2
(PPBP/CXCL7) |
|
NT-3
|
NT-4
|
Osteoprotegerin
(TNFRSF11B) |
PARC
(CCL18) |
PLGF
|
|
TGF beta 2
|
TGF beta 3
|
TIMP-1
|
TIMP-2
|
MIP-3 alpha
(CCL20) |
|
IP-10
(CXCL10) |
||||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C4 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
36
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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