人凋亡因子抗体芯片1
更新：2014/7/21 12:05:01 点击 ：1861
- 产品型号 AAH-APO-1
- 产品描述
Raybiotech Products
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
bad
|
bax
|
bcl-2
|
bcl-w
|
BID
|
|
BIM
|
Caspase-3
|
Caspase-8
|
CD40
(TNFRSF5) |
CD40 Ligand
(TNFSF5) |
|
cIAP-2
|
Cytochrome C
|
DR6
(TNFRSF21) |
Fas
(TNFRSF6/Apo-1) |
Fas Ligand
(TNFSF6) |
|
HSP27
|
HSP60
|
HSP70
|
HTRA2
|
IGF-1
|
|
IGF-2
|
IGFBP-1
|
IGFBP-2
|
IGFBP-3
|
IGFBP-4
|
|
IGFBP-5
|
IGFBP-6
|
IGF-1 R
|
livin
|
p21
|
|
p27
|
p53
|
SMAC
|
Survivin
(BIRC5) |
TNF RI
(TNFRSF1A) |
|
TNF RII
(TNFRSF1B) |
TNF alpha
|
TNF beta
(TNFSF1B) |
TRAIL R1
(TNFRSF10A/DR4) |
TRAIL R2
(TNFRSF10B/DR5) |
|
TRAIL R3
(TNFRSF10C) |
TRAIL R4
(TNFRSF10D) |
XIAP
|
||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Apoptosis Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- Protease Inhibitor Cocktail
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
43
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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