小鼠血管生成抗体芯片1
更新 ：2014/7/20 20:34:46 点击：1775
- 产品型号 AAM-ANG-G1
- 产品描述
Raybiotech Antibody Arrays
产品介绍
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
|
Scroll over each target protein for more information |
||||
|
Eotaxin-1
(CCL11) |
Fas Ligand
(TNFSF6) |
bFGF
|
GCSF
|
GM-CSF
|
|
IFN-gamma
|
IGF-2
|
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
IL-12 p40/p70
|
|
IL-12 p70
|
IL-13
|
IL-6
|
IL-9
|
Leptin
|
|
MCP-1
(CCL2) |
M-CSF
|
MIG
(CXCL9) |
Platelet Factor 4
(CXCL4) |
TIMP-1
|
|
TIMP-2
|
TNF alpha
|
Thrombopoietin
(TPO) |
VEGF-A
|
|
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Angiogenesis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
24
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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