人血管生成抗体芯片2
更新：2014/7/20 20:28:38 点击：1824
- 产品型号 AAH-ANG-G2
- 产品描述
Raybiotech Antibody Arrays
产品介绍
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
|
Scroll over each target protein for more information |
||||
|
Angiopoietin-1
|
Angiopoietin-2
|
Angiosestatin
|
Endostatin
|
GCSF
|
|
GM-CSF
|
I-309
(TCA-3/CCL1) |
IL-10
|
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
|
IL-2
|
IL-4
|
I-TAC
(CXCL11) |
MCP-3
(MARC/CCL7) |
MCP-4
(CCL13) |
|
MMP-1
|
MMP-9
|
PECAM-1
(CD31) |
Tie-2
|
TNF alpha
|
|
uPAR
|
VEGFR2
|
VEGFR3
|
||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Angiogenesis Antibody Array G2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
23
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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