人肥胖因子抗体芯片1
更新：2014/7/20 18:00:39 点击 ：1911
- 产品型号 AAH-ADI-1
- 产品描述
Raybiotech
产品介绍
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
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|
4-1BB
(TNFRSF9/CD137) |
ACE-2
|
Adiponectin
(ACRP30) |
Adipsin
(Complement Factor D) |
AgRP
|
|
Angiopoietin-1
|
Angiopoietin-2
|
ANGPTL4
|
CRP
(C-Reactive Protein) |
ENA-78
(CXCL5) |
|
Fas
(TNFRSF6/Apo-1) |
FGF-6
|
Growth Hormone
|
HCC-4
(CCL16) |
IFN-gamma
|
|
IGFBP-1
|
IGFBP-2
|
IGFBP-3
|
IGF-1
|
IGF-1 R
|
|
IL-1 R4
(ST2) |
IL-1 R1
|
IL-10
|
IL-11
|
IL-12 p70
|
|
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
IL-6
|
IL-6 R
|
IL-8
(CXCL8) |
|
Insulin
|
IP-10
(CXCL10) |
Leptin R
|
Leptin
|
LIF
|
|
Lymphotactin
(XCL1) |
MCP-1
(CCL2) |
MCP-3
(MARC/CCL7) |
M-CSF
|
MIF
|
|
MIP-1 beta
(CCL4) |
MSP alpha/beta
|
Osteoprotegerin
(TNFRSF11B) |
Oncostatin M
|
PAI-1
|
|
PARC
(CCL18) |
PDGF-AA
|
PDGF-AB
|
PDGF-BB
|
RANTES
(CCL5) |
|
Resistin
|
SAA
(Serum Amyloid A) |
SDF-1 alpha
(CXCL12 alpha) |
TNF RII
(TNFRSF1B) |
TNF RI
(TNFRSF1A) |
|
TECK
(CCL25) |
TGF beta 1
|
TIMP-1
|
TIMP-2
|
TNF alpha
|
|
VEGF-A
|
XEDAR
|
|||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Obesity Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
62
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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